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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Am J Transplant. 2018 May 15;18(10):2429–2442. doi: 10.1111/ajt.14870

Figure 1. Workflow of the study.

Figure 1

Forty allograft biopsy specimens obtained from 40 unique kidney allograft recipients were RNA sequenced. For each sample: (i) Biopsy infiltration score was derived as the sum of Banff acute scores, reported by the transport pathologist; (ii) A purity score was computed using the aligned RNA sequencing reads as input in ESTIMATE software; and (iii) Het/Hom ratio was computed after variant calling was done from the RNA sequencing data. Throughout the manuscript, the ratio of heterozygous to non-reference genome homozygous variants on all autosomes, for each sample, is called as the Het/Hom ratio, and the ESTIMATE score and 1-Purity score are used interchangeably.