Table 1.
Characteristics of kidney transplant recipients and RNA samples
| Kidney Allograft Biopsies (N=40) | |||||
|---|---|---|---|---|---|
| Characteristics | Acute T Cell Mediated Rejection |
Active Antibody Mediated Rejection |
Chronic Active Antibody Mediated Rejection |
Interstitial Fibrosis/ Tubular Atrophy |
Normal |
| N=11 | N=7 | N=11 | N=6 | N=5 | |
|
| |||||
| At the time of transplant | |||||
| Recipient information | |||||
| Age, years, mean (SD) | 51 (14) | 43 (15) | 51 (13) | 47 (11) | 45 (12) |
| Women, n (%) | 5 (45) | 4 (57) | 6 (55) | 2 (33) | 3 (60) |
| Racial categories, Black, n (%) | 3 (27) | 3 (43) | 2 (18) | 0 (0) | 0 (0) |
| Previous transplants, n (%) | 1 (9) | 0 (0) | 1 (10) | 0 (0) | 0 (0) |
| Donor information | |||||
| Age (years), mean (SD) | 44 (17) | 40 (18) | 44 (13) | 50 (7) | 40 (17) |
| Women, n (%) | 9 (82) | 3 (43) | 5 (46) | 2 (33) | 4 (80) |
| Racial categories, Black, n (%) | 1 (9) | 1 (14) | 1 (9) | 0 (0) | 0 (0) |
| Deceased donor, n (%) | 4 (36) | 2 (29) | 4 (36) | 5 (83) | 2 (40) |
| HLA-ABDR mismatch, median (IQR) | 4 (2.5–5) | 4.5 (3–5) | 4 (3–5) | 3 (3–4.5) | 5 (2–6) |
| Induction therapy, n (%) | 11 (100) | 7 (100) | 10 (91) | 3 (50) | 5 (100) |
| Antithymocyte globulin, n (%) | 8 (73) | 6 (86) | 10 (91) | 3 (100) | 5 (100) |
| After transplant and before the index biopsy | |||||
| Calcineurin inhibitors, n (%) | 11 (100) | 7 (100) | 11 (100) | 6 (100) | 5 (100) |
| Corticosteroids maintenance, n (%) | 3 (27) | 1 (14) | 9 (82) | 4 (67) | 0 (0) |
| At the time of index allograft biopsy | |||||
| Reason for biopsy, clinically indicated: surveillance, n | 11:0 | 7:0 | 11:0 | 6:0 | 0:5 |
| Time from transplantation to biopsy, months, median (IQR) | 13.5 (4.4–53.6) | 0.9 (0.4–15.3) | 62 (25–76) | 78 (43–108) | 6.3 (6.2–6.4) |
| Biopsy within 12 months of transplantation, n (%) | 4 (36) | 4 (57) | 1 (9) | 0 (0) | 5 (100) |
| Serum creatinine, mg/dl, median (IQR) | 2.10 (1.71–3.06) | 3.36 (2.51–3.70) | 1.71 (1.45–2.51) | 3.95 (3.00–5.09) | 1.20 (1.00–1.50) |
| RNA | |||||
| Quantity of total RNA from biopsy tissue, µg, median (IQR) | 2.45 (1.64–2.77) | 1.77 (1.27–1.95) | 1.95 (1.44–2.15) | 1.21 (0.90–1.48) | 1.18 (0.89–1.35) |
| Purity of RNA, OD260/OD280 ratio, median (IQR) | 2.11 (2.07–2.19) | 2.03 (2.02–2.36) | 2.03 (1.97–2.11) | 1.96 (1.88–1.99) | 2.02 (1.96–2.02) |
| RNA integrity number, median (IQR) | 7.2 (7.0–7.9) | 7.7 (7.3–7.8) | 7.9 (7.7–8.1) | 7.1 (6.7–7.9) | 7.2 (6.6–7.3) |
| Quantity of total RNA used for mRNA sequencing, ng | 400 | 400 | 400 | 400 | 400 |