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. 2018 Jun 1;37(39):5305–5324. doi: 10.1038/s41388-018-0294-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Effects of DKK3 silencing on TGFBI and ECM-1 protein and mRNA levels in WPMY-1 and RWPE-1 cells. a Western blots of CM from equal numbers of shCTRL (PSM2) and shDKK3 (Wsh8) WPMY-1 cells cultured in serum-free medium for 48 h were probed for TGFBI; a Coomassie Blue (CB)-stained gel of samples run in parallel was used as a loading control. b Densitometry analysis of TGFBI in CM; graph shows average intensity normalized to PSM2, error bars show SD, **p < 0.01, n = 5. c q-RT-PCR analysis of TGFBI mRNA levels, relative to 36B4, in shCTRL (PSM2/PSM3) and shDKK3 (Wsh7/Wsh8) WPMY-1 cells; error bars show SD. d Western blots of CM from equal numbers of shCTRL (NS11) and shDKK3 (sh6) RWPE-1 cells cultured in serum-free medium for 48 h were probed for TGFBI; a CB-stained gel of samples run in parallel was used as a loading control. e Densitometry analysis of TGFBI in CM; graph shows average intensity normalized to NS11, error bars show SD, **p < 0.01, n = 3. f q-RT-PCR analysis of TGFBI mRNA levels, relative to 36B4, in shCTRL (NS11) and shDKK3 (sh6) RWPE-1 cells; error bars show SD, n = 3, *p < 0.05. g–l ECM-1 protein and ECM1 mRNA levels were analyzed as in a–f; h **p < 0.01, n = 6, k *p < 0.05, n = 3