Fig. 1.

Loss of Ote causes germline stem cell-specific NL defects. a Schematic of the Drosophila nuclear lamina (NL). Underneath the inner nuclear envelope (yellow) resides the NL (blue) that contains three Drosophila LEM-D proteins, Otefin (Ote), Bocksbeutel (Bocks α and β), and dMAN1. Each protein localizes to the NL using a domain (orange, Peripheral localization, PL) that interacts with lamin or using transmembrane domains (orange, TM). Proteins in the LEM-D family carry a LEM-D (blue) that binds Barrier-to-Autointegration Factor (BAF, green), to promote chromatin assembly at the nuclear periphery. b Confocal images of ote^+/+ and ote^−/− (ote^B279G/PK) nuclei stained with antibodies against Lamin Dm0 (red) and Heterochromatin protein 1a (HP1a; green). Scale bars, 2.5 μm