Fig. 3.

Select DDR components contribute to the checkpoint pathway in ote mutants. a Schematic representation of signaling through the DNA damage response (DDR) pathway. Common triggers that activate the ATM and ATR responder kinases are shown. The responder kinases canonically activate the Chk2 and Chk1 transducer kinases, which signal to p53 and cause cell death. b Quantification of peak fecundity (eggs per female per day) of ddr, ote double-mutant females crossed to ote^+/+ males. Genotypes are noted below each bar, with ote^−/− corresponding to ote^B279G/PK. Error bars represent the standard deviation from a minimum of two independent experiments, each using 7–15 females. c Fecundity (eggs per female per day) of ddr, ote double-mutant females of indicated genotypes, crossed to wild-type males, with ote^−/− corresponding to ote^B279G/PK. Genotypes are noted above the graph. Error bars indicate the standard deviation from a minimum of three independent experiments with 7–15 females each. Wild-type data from Fig. 2c. were included in the graph as a reference. d. Quantification of germarial phenotypic classes (noted to the right) in 2-day-old ddr, ote double-mutant females, with ote^−/− corresponding to ote^B279G/PK. Number of germaria assessed from at least ten females is noted above each bar