Fig. 7.

Checkpoint inhibition rescues male GSC, but not somatic defects in ote mutants. a Confocal images of testes from 6-day-old males stained for Vasa (red) and Spectrin (green). Genotypes are noted above the images. The GSC niche is denoted by an asterisk. b Percentage of fertile 12–15-day-old males mated to ote^+/+ virgin females. Males were determined to be fertile if greater than 5 offspring were produced over a 3-day mating period as described in ref. ¹⁶. Genotypes are noted below, and the number of males analyzed is noted above each data set. Error bars indicate the standard deviation from a minimum of three independent experiments. c Confocal images of the central nervous system and surrounding imaginal discs dissected from wandering third instar larvae stained for DAPI (gray) and antibodies against phosphorylated serine 10 of Histone H3 (H3 S10ph; red). Genotypes are noted above each image. All scale bars are 100 µm. d Percent expected class from the following cross: y¹,w^67c23; chk2^P6,ote^PK/CyO; bocks^Δ66/Tm6b,Tb¹ crossed to y¹,w^67c23; chk2^P30,ote^B279G/CyO; bocks^Δ10/Tm6b,Tb¹. The genotype of the progeny is noted below and the number of progeny eclosed over the expected number is noted above