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. 2018 Sep 27;8:14491. doi: 10.1038/s41598-018-32820-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlative LM-EM imaging of a dendritic spine and its presynaptic partner. (A) A single confocal light microscopy image of a dendrite labeled with AVC. The arrow indicates the spine reconstructed in (C,D). (A’) An electron micrograph corresponding to the area show in (A). The area surrounded by a rectangle is shown in (B). (B) Magnification of the area indicated by a rectangle in (A’). The area surrounded by a rectangle is shown in (D). Arrows in (A’,B) indicate AVC labeled neuron. (C) The structure of a spine shown in (A) reconstructed from serial electron micrograph. Green; labeled spine, Red; its presynaptic partner. (D) Serial electron micrograph of the area indicated in (B). (D’) The DAB staining is highlighted with green and the presynaptic partner of the labeled spine is highlighted with red. Synaptic vesicles in the presynaptic bouton are highlighted with blue.