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. 2018 Sep 27;8:14452. doi: 10.1038/s41598-018-32744-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Basal and average metabolic heat production rates. (a–c) Basal heat production as measured for: (a) the three Drosophila genotypes considered: Canton S, w¹¹¹⁸, and yw, (b) Canton S flies of different ages, and (c) Canton S flies on high calorie (HCD), normal (ND) and restricted diets (RD) at different ages. (d–f) Mass-specific basal metabolic rate of flies (see Supplementary Fig. S2) of different: (d) genotypes, (e) ages, and (f) dietary conditions. (g–i) Mass-specific average heat production for the same flies as in (a–c). Shown are the mean (horizontal line), standard error (box) and standard deviation (error bars). The open circles to the left of the boxes represent the average heat production for individual flies and N indicates the sample size. ANOVA results are indicated on each panel; Tukey’s tests are indicated pairwise: ^*p < 0.01, ^**p < 0.005, ^***p < 0.001.