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. 2018 Sep 27;9:3963. doi: 10.1038/s41467-018-06314-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The lipid II–nisin complex in cellular membranes. a Comparison of ¹H-detected 2D ¹⁵N–¹H spectra of lipid II-bound nisin in native M. flavus membranes (magenta) and in DOPC (cyan). The gray spectrum shows nisin non-specifically bound to DOPG:DOPC liposomes (7:3 ratio) in the absence of lipid II. b Overlay of 2D ¹⁵N–¹H spectra of lipid II-bound nisin acquired in zwitterionic DOPC (cyan) and anionic DOPG:DOPC (7:3 ratio) (pink) liposomes. c Overlay of 2D ¹⁵N–¹H spectra of lipid II-bound nisin acquired in C18:1 DOPC (cyan) and C14:1 DMoPC (pink) liposomes. d ¹H/²H exchange: 2D ¹⁵N–¹H spectra of lipid II-bound nisin acquired in DOPC in fully protonated (cyan) and deuterated (red) buffers. e ¹⁵N R_1rho relaxation rates of lipid II-bound nisin acquired in DOPC. The error bars indicate the standard deviation of the fit. f Differential ¹⁵N T_1rho times comparing dynamics in C18:1 DOPC and C14:1 DMoPC liposomes. g ¹H/²H ssNMR exchange results. Dark and light blue beads represent residues that showed complete and intermediate exchange in deuterated buffers, respectively. Residues S29/I30 and U33/K34 could not be analyzed due to spectral overlap and fast dynamics, respectively