Figure 1.

Vika recombines specifically its target sites (vox) in mES cells. (a) Schematic diagram of the Rosa26-vox-neo-vox-lacZ reporter before and after Vika recombination. Two mESC clones (#3 and #9) with stably integrated lacZ reporters were transiently transfected with CAG-Vika-IRES-puro and analysed at 24 h and 48 h by lacZ staining. (b) Each of the four recombinase expression vectors (3 µg each per 6-well) were transfected in the four stable Rosa26 reporter mES cells lines. Cells were analysed 48 h after transfection by lacZ staining. (Scale bar = 100 µm).