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. 2018 Sep 27;8:14453. doi: 10.1038/s41598-018-32802-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The multi site reporter vector is functional in mESCs. (a) Schematic diagram of the Rosa26-multi site reporter (MuX) before and after Vika, Flpo, Dre and Cre recombination. Stable mESC reporter clones were transiently transfected with expression vectors and analysed at 48 h for GFP expression. (b) Flow cytometry to measure GFP levels after transfecting different amounts of the recombinase expression vectors (0.05 to 1 µg per 6-well). Representative plots of cells transfected with 1 µg recombinase expression vector are shown. (c) Quantification of (b). Error bars represent standard deviations. Statistical significance is shown only for P < 0.01 (**) and P < 0.001 (***).