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. 2018 Sep 27;8:14457. doi: 10.1038/s41598-018-32780-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced percentage of thymocytes in PON1^−/− rats. (a) Images of the thymus from 2, 6 and 10-month-old PON1^−/− and PON1^+/+ rats (n = 5 per group). (b) Weight of the thymus from PON1^−/− and PON1^+/+ rats aged 2-months (n = 9 per group), 6-months (n = 6 per group) and 10-months (n = 5 per group) old. (c) Hematoxylin and eosin staining of thymus from PON1^−/− and PON1^+/+ rats aged 2-months, 6-months and 10-months (n = 3 per group). Scale bar = 200 μm. (d) Representative histograms of T cells numbers in the spleen, peripheral blood (PB), and thymus from PON1^−/− and PON1^+/+ rats aged 2 months. (e) Flow cytometric analysis of the percentage of T cells in the spleen, PB, and the thymus from 2-month-old PON1^−/− and PON1^+/+ rats. Data represent the mean ± SD of three independent experiments (n = 5 per group). *P < 0.05; **P < 0.01.