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. 2018 Sep 27;8:14446. doi: 10.1038/s41598-018-32758-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Optical micrographs of iBATs stained with silver-impregnation (a–c), and TEM images of iBAT stained with Os (d–f) for a normal mouse (PLPEG-CNT−, no fasting) (a,d), a mouse (PLPEG-CNT−, fasting18h+, Refeeding2h−) (b,e), and a mouse (PLPEG-CNT−, fasting18h+, Refeeding2h+) (c,f). Reticular fibers (type III collagen) was stained black with silver impregnation. Black arrow heads point the inter-cellular spaces. (n = 2) (B) TEM of iBAT of a mouse (PLPEG-CNT−, fasting18h+, Refeeding2h+). An end of the collagen band (a,b, red arrow head), narrow part of collagen (c, black arrow head), and the gap junction (d–f), showing its end (f, blue arrow head) (n = 1).