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. 2018 Sep 27;8:14446. doi: 10.1038/s41598-018-32758-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NIR fluorescence micrographs of aBAT-LNs of a mouse (PLPEG-CNT+, Fasting20h+, Refeeding3h+; PIT 5 h) observed under different magnification (a–c). CNT accumulating areas are brightly imaged. Fluorescence intensities of lymph nodes in aBAT and ingWAT embedded in paraffin blocks (d). The tissues were taken at PIT 5 h. Back ground fluorescence intensity of 1500 was subtracted (n = 5).