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. 2018 Sep 27;8:14493. doi: 10.1038/s41598-018-32861-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of certain marker proteins in the uptake of exo-N and exo-S2 by donor cells B16BL6 and other allogeneic cells C26. Labeled exo-N (A and B) or exo-S2 (C and D) were incubated with different Abs in a ratio 1:1 for 2 h at 4 °C and then added to different cancer cell lines, namely B16BL6 (A and C) and C26 (B and D). After 4 h incubation, cancer cells were harvested for analysis by flow cytometry. All data represent the mean ± SD of triplicates. An unpaired t test was applied for each value relative to untreated cancer cells, asterisks indicate different levels of significant difference; *p < 0.05 and **p < 0.01.