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. 2018 Sep 27;8:14493. doi: 10.1038/s41598-018-32861-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of temperature on exosome (EV) uptake by donor cells (B16BL6) and allogeneic cells (C26). Labeled samples of exo-N and exo-S2 were incubated with different cancer cell lines at 4 °C or 37 °C. After 2 h incubation, cancer cells were harvested for analysis by flow cytometry (A) or imaged by laser scanning confocal microscope after staining the DNA core with Hoechst 33342 (B). All data represent the mean ± SD (A) and one set (B) of triplicates. An unpaired t test was applied for each value relative to untreated cancer cells (***p < 0.001). Exosomes (EVs) were labeled with PHK67 (green) and the DNA core was stained with Hoechst 33342 (blue). Scale bar indicates 20 µm.