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. 2018 Sep 27;8:14493. doi: 10.1038/s41598-018-32861-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uptake mechanisms for the internalization of exo-N and exo-S2 by donor cells B16BL6 and other allogeneic cells C26. B16BL6 (A and C) and C26 (B and D) cancer cell lines were incubated in the presence of different uptake inhibitors for 30 min and then labeled exo-N (A and B) or exo-S2 (C and D) were added. After 4 h incubation, cancer cells were harvested for analysis by flow cytometry. All data represent the mean ± SD of triplicates. An unpaired t test was applied for each value relative to untreated (NT) cancer cell, asterisks indicate different levels of significant difference; *p < 0.05, **p < 0.01 and ***p < 0.001. NT, untreated cancer cells.