. 2018 Sep 27;8:14493. doi: 10.1038/s41598-018-32861-w

Table 1.

Identification of exosomal markers via shotgun analysis.

	Protein name	Protein hits	exo-N	exo-S1	exo-S2
		Protein hits	404	258	552
		Accession number	MW (KDa)	pI	Score (peptide matches)
		Accession number	MW (KDa)	pI	exo-N	exo-S1	exo-S2
Tetraspanins	CD9	gi\|388912	25.241	6.88	34	0	79
	CD63	gi\|976238	25.479	6.69	42	35	99
	CD81 (Tapa-1 protein)	gi\|8574076	25.797	5.54	421	394	453
	CD82	gi\|148695678	22.373	7.98	114	68	93
	CD151 (SFA-1)	gi\|2447007	28.257	7.44	89	62	73
Heat shock proteins	HSPA8 (HSP70.1)	gi\|118490060	70.088	5.53	186	107	173
Heat shock proteins	HSP90 (Endoplasmin)	gi\|119362	92.418	4.74	117	117	115
Enzymes	Lactate dehydrogenase A (LDHA)	gi\|126048	36.475	7.74	79	0	26
	GAPDH	gi\|120702	35.787	8.44	292	226	644
	Alpha enolase 1 (Eno-1)	gi\|12832241	47.111	6.37	259	155	454
	Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein (YWHAE or Phopholipase A2)	gi\|1304166	27.754	4.73	109	133	138
	L-lactate dehydrogenase B chain isoform Ldhbx	gi\|938085832	37.325	5.85	119	92	82
	L-lactate dehydrogenase B chain isoform 2	gi\|718551069	27.989		119	85	73
	Fructose-bisphosphate aldolase B	gi\|15723268	39.548	8.52	48	79	52
	Phosphoglycerate kinase (PGK1)	gi\|202423	44.508	7.53	108	75	345
	Chain A, Modified Glutathione S-Transferase (Pi) Complexed With S (P- Nitrobenzyl)glutathione	gi\|4557944	23.471	7.85	107	164	147
Others	Flotillin-1	gi\|2149604	47.484	6.71	50	0	86
	Annexin-A2	gi\|113951	38.652	7.55	33	0	42
	Epidermal growth factor (EGF)	gi\|49523319	122.888	6.79	39	0	24
	lysosomal membrane glycoprotein A (LAMP-2)	gi\|293693	45.618	7.05	42	0	67
	NPC1	gi\|2251242	142.795	5.52	42	0	64
	Clathrin (light chain)	gi\|34785471	23.618	4.43	116	0	116
	TSG101	gi\|3184260	44.096	6.28	153	121	184
	Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)	gi\|13278546	50.082	9.1	187	85	255
	Programmed Cell Death 6 Interacting Protein (PDCD6IP)	gi\|30048422	96.251	6.15	631	219	537
	Albumin (ALB)	gi\|3647327	68.648	5.75	550	504	878
	Gamma actin	gi\|6425087	43.572		264	162	451
	Cofilin-1 (CFL1)	gi\|116849	18.548	8.22	175	48	142
	Ferritin heavy chain	gi\|309233	21.086	5.62	53	41	50
	Alpha-4 integrin	gi\|1173604	115.013	6.29	294	381	538
	Clathrin (heavy chain)	gi\|51491845	191.435	5.48	327	96	1768
	Lactadherin (MFGE8)	gi\|113865979	51.208	6.11	426	80	398