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. 2018 Sep 27;9(10):1008. doi: 10.1038/s41419-018-1050-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Jurkat cells were transfected for 48 h either with non-targeting control (ANC) or synthetic miR-34a-5p mimic. Respective Ca²⁺ imaging was performed in five independent experiments on 3 days of measurement. Intracellular Ca²⁺ was sensed by Fura-2-AM fluorescent dye and SOCE was induced by Thapsigargin (Tg). Cells were perfused with different solutions providing external Ca²⁺ ([Ca²⁺]_ext). a Quotient of Fura-2 fluorescence (Ratio (340/380)) was determined over time of measurement as mean of all tested cells (ANC: n = 710; miR-34a-5p: n = 756). b Ratio (340/380) and delta ratio (340/380) were determined for the functional sections of imaging procedure and evaluated as mean for each of the five experiments. Results were standardized to controls and are shown as means of all experiments with corresponding standard errors (SEM). Statistical evaluation was performed using Student’s t-test. A normal distribution of the data was assumed. (*p ≤ 0.05)