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. 2018 Sep 27;9(10):1008. doi: 10.1038/s41419-018-1050-7

Fig. 4. Western blot analysis of endogenous NFATC4, STIM1, and PPP3R1 protein levels in miR-34a-5p-overexpressing Jurkat cells.

Fig. 4

a, b Representative western blot images. Jurkat cells were transfected for 48 h either with non-targeting control (ANC) or synthetic miR-34a-5p mimic. NFATC4, STIM1, and PPP3R1 protein was detected by specific monoclonal antibodies in western blot analysis. β-Actin served as loading control. c–e Quantification of NFATC4, STIM1, and PPP3R1 expression by densitometric analysis of three independent western blot experiments. Respective protein expression was standardized according to β-Actin loading control and expression level of the control transfected cells was set to 100%. Results are shown as means of five independent experiments with corresponding standard errors (SEM). Statistical evaluation was performed using Student’s t-test. A normal distribution of the data was assumed. (*p ≤ 0.05, **p < 0.01, ***p < 0.001; PPP3R1 protein phosphatase 3 regulatory subunit B alpha, NFATC4 nuclear factor of activated T cells 4, STIM1 stromal interaction molecule 1