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. 2018 May 30;7(3):264–270. doi: 10.1016/j.imr.2018.05.003

Table 2.

The List and Sequence of Primers Used for RT-PCR Analysis

No. Gene Forward primer (5′–3′) Forward primer (3′–5′) Annealing temperature (°C)
1 Kitl GGTAGCCAGGAGTTTGTTCT TTGTGTGGCATAAGGGCT 58
2 Cyp11a1 TCCTCAAAGCCAGCATCA ATCTCGACCCATGGCAAA 58
3 Ptgs2 ACCTCTCTGAACTATGGTGT TGCAGTCTGCTTTATGCG 58
4 Actin TACGTCGCCCTGGATTTT ATGAAAGAGGGCTGGAAGAG 60