Table 2.
No. | Gene | Forward primer (5′–3′) | Forward primer (3′–5′) | Annealing temperature (°C) |
---|---|---|---|---|
1 | Kitl | GGTAGCCAGGAGTTTGTTCT | TTGTGTGGCATAAGGGCT | 58 |
2 | Cyp11a1 | TCCTCAAAGCCAGCATCA | ATCTCGACCCATGGCAAA | 58 |
3 | Ptgs2 | ACCTCTCTGAACTATGGTGT | TGCAGTCTGCTTTATGCG | 58 |
4 | Actin | TACGTCGCCCTGGATTTT | ATGAAAGAGGGCTGGAAGAG | 60 |