Figure 8.

RF-EMR alters sperm motility but not tail tyrosine phosphorylation levels. Mature mouse spermatozoa isolated from the cauda epididymis were exposed to RF-EMR of an intensity of 0.15 W/kg for periods of up to 4 h. At regular intervals during exposure, computer assisted sperm analysis was performed for parameters of sperm motility. (A) Sperm total, (B) progressive, (C) rapid motility, and (D) straight-line velocity. (E) Spontaneous sperm tyrosine phosphorylation levels were assessed via immunoblotting. Three replicates were performed for both control and RF-EMR treated sperm protein extracts. The intensity of each lane was then quantified via pixel intensity (F). The entire lane was quantified relative to hexokinase, as the loading control (arrow). These analyses were performed on at least three biological replicates. *p < 0.05 compared to unexposed controls.