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. 2018 Sep 21;9:1059. doi: 10.3389/fphar.2018.01059

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Copyright © 2018 Wu, Chen, Tan, Fan, Xie, Zhang, Chen, Li, Liu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of TGFBR1 partially blocks the inhibitory effect of luteolin on vascular smooth muscle cell (VSMC) migration. (A) A7r5 and HASMC cells were incubated with TGFBR1 overexpression adenovirus vector and GFP control adenovirus for 48 h and then treated with luteolin (40 μM) for 24 h. Then, the migration of VSMCs was tested by wound healing assays. (B,C) Percentage of relative VSMC migration (n = 3). (D) A7r5 and HASMC cells were incubated with TGFBR1 overexpression adenovirus vector and GFP control adenovirus for 48 h. Then, Transwell assays were performed after treatment with luteolin (40 μM) for 12 h. (E,F) The number of cells in each field of view (n = 5). Data are shown as the mean ± SD. ^∗P < 0.05, ^∗∗P < 0.01.