Figure 1.

Leptin triggers PGD₂ and LTC₄ synthesis, but not PGE₂, within eosinophils. Human eosinophils (A–C) were stimulated with PAF (1 μM), hrCCL5 (100 ng/mL), or hrleptin (50 nM) for 1 h. Mouse eosinophils (B–F) were stimulated with mrleptin (50 nM) for 1 h. Eicosanoid PGE₂, PGD₂, or LTC₄ production by eosinophils were quantified in cell supernatants by specific EIA kits. Values are expressed as the mean ± SEM of at least three distinct donors or three different mouse bone marrow cultures. + p < 0.05 compared with non-stimulated eosinophils.