Figure 2.

Leptin triggers lipid body biogenesis within eosinophils. In (A) human eosinophils were stimulated with PAF (1 μM), hr CCL5 (100 ng/mL), or hr leptin (0.5–50 nM) for 1 h or 15 min, as indicated. (B) shows lipid bodies stained by Oil Red O within eosinophils stimulated with hr leptin (50 nM) for 1 h. In (C) mouse eosinophils were stimulated with mr leptin (50 nM) for 1 h. In (A) and (C) lipid bodies were enumerated in 50 consecutive osmium-stained cells. Values are expressed as the mean ± SEM of at least three distinct donors or three different mouse bone marrow cultures. + p < 0.05 compared with non-stimulated eosinophils.