Figure 6.

Endogenous eosinophil-derived PGD₂ mediates leptin-induced lipid body-driven LTC₄ synthesis. In (A) human eosinophils were pretreated with PGD₂ synthesis inhibitor HQL-79 (10 μM) for 30 min before stimulation with hr leptin (50 nM) for 1 h. In (B) mouse eosinophils were pretreated with PGD₂ synthesis inhibitors HQL-79 (10 μM) or AT-56 (10 μM) for 30 min before stimulation with mr leptin (50 nM) for 1 h. Lipid body count was evaluated in osmium-stained cells and LTC₄ production in cell-free supernatants by EIA kits. Values are expressed as the mean ± SEM of at least three distinct donors or three different mouse bone marrow cultures. + p < 0.05 compared with control. ^* p < 0.05 compared with leptin-stimulated eosinophils.