Figure 7.

Autocrine CCR3 activation by eosinophil-derived CCL5 elicits PGD₂ synthesis in response to leptin stimulation of eosinophils. In (A) mouse eosinophils were stimulated for 1 h with PGD₂ (25 nM) or mr leptin (50 nM); mr leptin-stimulated cells were pretreated LY294002 (10 μM) for 30 min. In (B) and (C) human eosinophils were pretreated with HQL-79 (10 μM) or neutralizing anti-CCR3 antibody (10 μg/mL) for 30 min before stimulation with respectively hr leptin (50 m nM) for 1 h. CCL5 secretion was evaluated in cell free supernatants by ELISAand PGD₂ production in cell-free supernatants by EIA kits. Values are expressed as the mean ± SEM of at least three distinct donors or three different mouse bone marrow cultures. + p < 0.05 compared with control. ^*p < 0.05 compared with leptin-stimulated eosinophils.