Figure 8.

Autocrine CCR3 activation does not play a role in eosinophil activation by PGD2. Human eosinophils were pretreated with neutralizing anti-CCR3 antibody (10 μg/mL) for 30 min before stimulation with PGD₂ (25 nM) for 1 h. Lipid body count was analyzed in osmium-stained cells and LTC₄ production in cell-free supernatants by EIA kits. Values of top panel are expressed as the mean ± SEM of at least three distinct donors. + p < 0.05 compared with control. In bottom panel, normalized values show mean ± SEM and individual % percentage of LTC₄ control levels, which were for each of the three donors analyzed 10, 75, and 656 pg/2 × 10⁶ human eosinophils.