Table 2.
Primers used for Yersinia species identification.
| Yersinia species | Gene name | Forward and reverse primers sequences (5′-3′) | Size of PCR product (bp) | Annealing temp (°C) |
|---|---|---|---|---|
| Y. enterocolitica | ail | ACTCGATGATAACTGGGGAG CCCCCAGTAATCCATAAAGG |
170 | 56 |
| Y. enterocolitica | gyrB | AACCGATCAAACCGGGACAA AGCTTGATTGAAACCCCGGA |
138 | 60 |
| Y. pseudotuberculosis | inv | CGGTACGGCTCAAGTTAATCTG CCGTTCTCCAATGTACGTATCC |
183 | 60 |
| Y. bercovieri | gyrB | CGCAAGCACCTCTGAAAGTG CCGTACGGAAACCAACCAGA |
414 | 60 |
| Y. mollaretii | gyrB | AACTGACCTGACCGGGACTA GGAAGCCCACCAAATGGGTA |
380 | 60 |
| Y. intermedia | gyrB | AAGTTTCTGGCGGTTTGCAC CAGATCGGTTTCACCCACCA |
138 | 60 |
| Y. aldovae | gyrB | GATCCGCCGTGACGGTAAAG GCCCTGATACCGCCTTCATA for nested-PCR: |
60 | |
| CGCAGGCACCACTGAAAATG TGACACACCCGAGTTCAGGA |
158 | 60 |