Table 1.
Differentially up- and down-modulated genes and proteins in the most reported human tolDC-inducing protocols.
| Protocol | Type | Up-modulated molecules | Down-modulated molecules | References | |
|---|---|---|---|---|---|
| tolDC | Dexamethasone | Gene | ANXA1, C1QA, C1QC, C1QTNF1, C3AR1, CCL17, CD163, CD300LF, CD32, CFH, CLIC2, CSGALNACT1, CTSC, DCR3, EP2, EP3, F13A, FCGR2A, FCGR2B, FKBP5, FOXO3, FPR1, GILZ, GPX1, IDO1, IL10, IL12A, IL27B, IMDH2, JAG1, MERTK, MRC1, MT1, NCF1, OSF1, P2RY14, SLC39A8, SOD2, STAB1, TPP1, ZBTB16 | CCL22, CD1C, FCER1A, IDO1, IL12B, LAMP3, MMP12, ZNF366 | (26, 32, 34–36, 38, 39, 41) |
| Protein | CYP1B1, DAB2, DPYD, FCER1G, FCGR3A, FTL, GCLC, IVNS1ABP, LRRC25, MCTP1, MERTK, NUDT16, PDCD4, PECAM1, RNASE6, RNASET2, SIGLEC5, SLCO2B1 | FSCN1 | (12, 34, 37) | ||
| miRNA | miR-328-5P, miR-638, miR-663, miR-762, miR-1275, miR-1228, miR-1909 | miR-142-5p | (40) | ||
| Dexamethasone + rosiglitazone | Gene | FABP4, GILZ | (47) | ||
| Dexamethasone + vitamin D2 | Protein | ERK1/2, IDO, JNK/SAPK, mTOR, p38 MAPK, STAT3 | (48) | ||
| Dexamethasone + vitamin D3 | Gene | ACADM, ACADVL, ACO1, ACO2, ACOX2, ACSS1, ALDH2, ATP5G3, ATP5J, ATP5O, BLVRB, C1orf162, C1QA, CCR5, CD14, CD209, CD274, CD52, CLIC1, COX11, COX6A1, COX7A2, CTSB, CTSD, CTSH, CYC1, DHRS9, EIF3B, EIF3C, EIF3CL, EIF4A3, FBP1, FCGR2B, FCGR3A, FN1, FTH1, FTL, G6PD, GAPDH, IDH3A, IDH3B, ILT3, LDHB, LILRB4, MATK, MCEMP1, MDH2, ME1, ME3, NDUFB9, NDUFS1, NDUFS8, NOS3, PCK2, PDHA1, PDXK, PIK3R1, PKM2, PNP, PRDX3, PTPN6, RAC2, RGCC, RPS12, RPS19, RPS21, RPS6KA1, RPS6KA2, SDHA, SLC11A1, SLC27A5, SLC2A1, SLC2A5, SNCA, SUCLG1, SUCLG2, TCEB1, TGFB1, TP53, TPI1, UQCR10, UQCR11, UQCRB, UQCRC1 | ACTB, ADAM12, ADAM19, ANKRD33B, AOC1, CD25, CD40, CD80, CD83, CD86, DPYSL2, EHF, FSCN1, GPR157, ICOSLG, IKZF1, IKZF4, IL12B, IL2RA, ORMDL3, PIK3CG, PLEKHA5, PPP1R16B, PTPN2, SH2B3, TYK2, WDR1 | (49–51) | |
| Protein | ADK, AKR1A1, ALDH2, ALDOA, ATP5H, ECHS1, FBP1, FTL, G6PD, GPD2, GALK, MPDH2, PGAM, PGM1, PKM2, PNP, PRDX6, TALDO1, TKT, TPI1 | DPYSL2, ENO1, FSCN1, HSPD1, PDIA3 | (37) | ||
| Hepatocyte growth factor | Gene | IL10 | (52) | ||
| IFN-γ | Gene | IRF4, RELB, IL12p40 | (53, 54) | ||
| IL-10 | Gene | ANXA1, C1QC, CTSB, CTSC, CTSL, F13A, FTH1, GILZ, HLA-DOB, IL8, LILRB3, MRC1, STAB1, THBS1, TPP1 | CD74, LAMP3 | (32, 41, 55) | |
| IL-10 + IL-6 | Gene | CTSB, CTSL, FTH1, HLA-DOB, IL-8, THBS1 | CD74 | (55) | |
| Poly I:C | Gene | IDO1, PDL1 | (56, 57) | ||
| Rapamycin | Gene | ANXA1, C1QC, CTSC, GILZ, GPX1, IMDH2, OSF1, TPP1 | RALDH1 | (32) | |
| Retinoic acid | Gene | ALDH1A1, ALDH1A2, CD141, GARP | (58, 59) | ||
| TGF-β | Gene | ANXA1, CTSL, CXCL1, CXCR3, FTH1, HLA-DOB, IL8, LILRB3, THBS1 | CD74, STAB1 | (32, 55) | |
| TX527 (vitamin D3 analog) | Protein | ACADVL, ACO2, ACOX1, ATP5A1, CTSD, CTSS, COPG, FBP1, G6PD, HADHA, IDH3A, MnSOD, OGDH, PCK2, PKM2, PRX3, PTM, UQCRFS1 | ACAT1, ARCN1, DLD, PA28beta, PTM, RabGDI | (60) | |
| Vitamin D3 | Gene | ALOX5, ATP5A1, CAMP, CCL22, CD14, CD300LF, CMYC, CYP24, CYP24A1, CYP27B1, GILZ, GLUT3, HK3, ILT3, IRF8, LDHA, LGALS9, PDHA1, PFKFB4, PIK3CG, PRKAA1, THBD, VDR | CD1A, CD1C, CD1E, CD36, CD80, F13A, IER3, IRF4, LAMP3 | (32, 36, 41, 61–63) | |
| Protein | AKT, FTL, GSK-3b, mTOR | FSCN1, SOD2 | (37) | ||
| miRNA | miR-378 | (64) |
Genes validated by qPCR or proteins validated by western blot are shown in bold.