Figure 1.

Dual localization of GCS/eYFP and PDF/eYFP after particle bombardment of Arabidopsis thaliana leaves. The coding sequences of GCS/eYFP (A) and PDF/eYFP (B) were transiently expressed under the control of the CaMV 35S promoter after particle bombardment of leaf epidermis cells of Arabidopsis thaliana and analyzed by confocal laser scanning microscopy. Representative cells showing dual localization of the candidate proteins in both mitochondria and chloroplasts are presented as overlay images of the chlorophyll channel (displayed in red) and the eYFP channel (displayed in yellow). The borders of the transformed cells are depicted by a continuous white line. The strong chlorophyll signals in the background are derived from the larger chloroplasts of untransformed mesophyll cells underneath the epidermal cell layers. The squares highlight areas of the transformed cells that are shown in higher magnification separately for the chlorophyll channel and the eYFP channel, as indicated. The position of representative plastids of each transformed cell is encircled for better visualization. Representative mitochondria are marked (M). The scale bars correspond to 10 μm.