Table 1.
Particle bombardment (Arabidopsis) | Protoplast transformation (Arabidopsis) | Agrobacterium infiltration (Nicotiana) | Transgenic plants (Arabidopsis) | Mass spectrometry**(Arabidopsis) | Literature data | |
---|---|---|---|---|---|---|
GrpE/eYFP (At5g55200*) | a) Dual b) Mito |
a) Dual | a) Dual b) Mito |
a) Mito | Mito | Dual (Onion)a Mito (Arabidopsis)a Dual (Pea)b |
EF-Tu/eYFP (At4g02930*) |
a) Mito b) Dual |
a) Dual b) Mito c) Aggregates |
a) Dual b) Mito |
a) Mito |
Mito Plastid |
Dual (Pea)b |
GCS/eYFP (At2g35370*) | a) Dual |
a) Dual b) Aggregates |
a) Dual | a) Dual |
Mito Plastid |
Dual (Pea)b |
PDF/eYFP (At5g14660*) | a) Dual | a) Dual | a) Dual | a) Dual | Plastid | Dual (Pea)b Dual (Onion)c |
mtRi/eYFP (GB:X79332.1*) |
a) Mito b) Cytosol + Nucleus |
Mito Cytosol + Aggregates |
Mito Cytosol |
a) Mito | – | Mito (Pea)b |
FNR/eGFP (GB:M86349.1*) | a) Plastid | a) Plastid b) Cytosol + Nucleus |
a) Plastid | a) Plastid | – | Plastid (Pea)b |
Dual, localization in mitochondria and plastids; Mito/Plastid, localization exclusively in mitochondria/plastids; Aggregates, protein aggresomes in the transformed cell.
a, b and c address distinct localization in different cells of the same experiment. Preferential or exclusive localization is highlighted in bold.
Accession number of the corresponding candidate gene;
Data obtained from MASCP GATOR (Joshi et al., 2011; Mann et al., 2013) and SUBA4 Databases (Hooper et al., 2017);
(Van Aken et al., 2009);
(Baudisch et al., 2014);
(Giglione et al., 2000).