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(A) Release mechanism studied by treatment with ATP, dATP and adenosine. (B) Morphological observation of CrossPPA/siRNA polyplex before and after 5 mM ATP treatment. (C) Charge reversal of 25k PEI, 1.8k PEI, PEI-PBA and CrossPPA with different amounts of ATP added. (D) Reaction scheme of the charge reversal of PEI-PBA with treatment of ATP.
