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(A) Luciferase gene silencing mediated by siLuc-loaded polyplexes at various w/w ratios. *p < 0.05, **p < 0.01, ***p < 0.001. (B) 4T1 tumor cell killing of siBcl2-loaded polyplexes. (C) Bcl2 protein expression level after treatment with siBcl2-loaded polyplexes and analysis by western blot. (D) Cell apoptosis analysis by Annexin V-FITC/PI double staining.
