Table 1.
Anti-HER3 IgG and antibody fragments binding kinetics.
| Antibody Format | Valency | Average Expression Yield (mg/L) | Average KD (nM) | Average kON (×104 M-1 s-1) | Average kOFF (×10-4 s-1) | Average KD of IRDye800CW -labeled antibody (nM) |
|---|---|---|---|---|---|---|
| scFv | Monovalent | 5 | 20 ± 1.4 | 3.3 ± 0.1 | 5.4 ± 0.3 | 29 ± 3.7 |
| Fab | Monovalent | 5 | 3.5 ± 0.1 | 4.9 ± 0.1 | 1.7 ± 0.1 | 3.2 ± 0.1 |
| diabody | Bivalent | 2 | 3.0 ± 0.2 | 15 ± 0.1 | 4.4 ± 0.3 | 2.4 ± 0.2 |
| scFv-CH3 | Bivalent | 39 | 0.5 ± 0.1 | 9.6 ± 0.1 | 0.5 ± 0.1 | 2.1 ± 0.1 |
| scFv-Fc | Bivalent | 49 | 0.6 ± 0.1 | 7.8 ± 0.2 | 0.5 ± 0.1 | 0.9 ± 0.1 |
| IgG | Bivalent | 4 | 0.3 ± 0.1 (31.8 ± 0.5)* |
2.6 ± 0.1 (0.5 ± 0.1)* |
0.10 ± 0.01 (1.7 ± 0.1)* |
0.5 ± 0.1 |
* Anti-HER3 IgG binding kinetics to murine HER3