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. 2018 Sep 9;8(17):4679–4694. doi: 10.7150/thno.26643

Figure 1.

Figure 1

PBMNCs are induced into neural stem cells by using Sendai virus. (A) A schematic representation of the neural stem cells induction procedure. PBMNCs: peripheral blood mononuclear cells. (B) Phase-contrast images of the PBMNCs, an iNSC clone, iNSCs in sphere and iNSCs in monolayer culture. Scale bars, 200 μm. (C) The growth curve of iNSCs P10, P20 and P30. (n=3 independent experiments). (D) Karyotype analysis of iNSCs. (E) Detection of remaining Sendai virus by PCR and immunocytochemistry staining. Scale bars, 50 μm. (F) Expression of transgenes (SOX2, OCT4, KLF4, cMYC) and GAPDH reference gene at different passages of iNSCs. The values represent mean ± SEM in this figure and all the others in this article.