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. 2018 Sep 9;8(17):4679–4694. doi: 10.7150/thno.26643

Figure 3.

Figure 3

Differentiation of iNSCs in vitro and in vivo. (A) Immunofluorescent staining of MAP2, GFAP, O1 and SYN on iNSC (P30)-differentiated cells. Scale bars, 50 μm. SYN: Synapsin. White arrowheads represent SYN/TUJ1-double positive cells. (B) Immunofluorescent staining of HNA, TUJ1, NEUN, KI67 and OCT4 in SCID-beige mouse brain one month after iNSC (P30) transplantation. Scale bars, 50 μm. HNA: human nuclei antibody. (C) Differentiation efficiency of iNSC1, iNSC2 and fetal NSCs (n=3 independent experiments). (D) Differentiation efficiency of iNSCs following transplantation into SCID-beige mice (n=3). P30: passage No. 30.