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In situ hybridization assays of four TaTCP genes. (A–D) TaPCF5; (E–H) TaPCF6; (I–L) TaTCP21; and (M–P) TaTCP18. Spike developmental stages were W3.5: A, E, I, M; W5.5: B, F, G, N; W6.5: C, G, K, O. Sense probes were used for D, H, L, and P. Glu, glume; Lem, lemma; Pal, palea; Pis, pistil; Sta, stamen; and bar = 500 μm.
