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. 2018 Sep 10;9:1282. doi: 10.3389/fpls.2018.01282

FIGURE 13.

FIGURE 13

Functional characterization of the wheat TaTCP9 gene. (A–D) In situ hybridization of TaTCP9 in immature seeds at 2 DAP (A), 4 DAP (B), and 6 DAP (C) in Chinese Spring. (D) sense control. Pe, percarp; VB, vascular bundle; NP, nucellar projection; EC, endosperm cavity; ETC, endosperm transfer cells; and NPTC, nucellar projection transfer cells. (E,F) Expression pattern of TaTCP9 during grain development in hexaploid wheat (E) and tetraploid wheat (F) Each point of the FPKM represents the average of three biological replicates. (G) the mutation sites on the TaTCP9-A gene that gave premature stop codons in two Kronos mutant lines L2431 and L3090. (H) grain morphology of the mutants L2431 and L3090 relative to the wild type Kronos. (I) Increased grain length in L2431 and L3090. Error bars indicate SD. A total of 23 spikes from six L2341 plants and 12 spikes from four L3090 plants were measured. Student’s t-test, ∗p < 0.05, ∗∗p < 0.01. Bars in A–D indicate 500 μm; Bar in H indicates 1 cm.