Figure 1.

Study schema and plasma viral loads. (A) Animals were inoculated with 2 × 10⁶ PFU/mL of Zika Virus (ZIKV) isolate PRVABC59 intravaginally (IVAG) (RFc15R, R1811R, and A12T006) and subcutaneously (sub Q) (RZi15R and R21612R). Blood samples were collected at baseline, 3, 5, 7, 14, 21, 28, 60, and 110 days postinfection (dpi). Animals were sacrificed at 8 dpi (A12T006), 21 dpi (RZi15R, R21612R), 60 dpi (R1811R), and 110 dpi (RFc15R). (B) ZIKV RNA was measured in the plasma by quantitative real-time polymerase chain reaction (qRT-PCR) on various time points as described in the figure above.