Table 3.

Determination of the protein binding ratio of methyl 3-amino-6-methoxythieno [2,3-b] quinolone-2-carboxylate (PU-48) in rat and human plasma (n = 5).

Species	Added Concentration (µg/mL)	Concentration in Plasma (µg/mL)	Concentration in Buffer (µg/mL)	Protein-Binding Ratio (%)
Rat	0.25	0.127 ± 0.007	0.012 ± 0.003	90.70 ± 2.18
	1	0.524 ± 0.032	0.047 ± 0.002	91.06 ± 0.78
	4	2.128 ± 0.166	0.194 ± 0.019	90.83 ± 1.17
Human	0.25	0.146 ± 0.008	0.012 ± 0.003	91.60 ± 1.57
	1	0.611 ± 0.027	0.052 ± 0.003	91.48 ± 0.64
	4	2.290 ± 0.093	0.231 ± 0.029	89.90 ± 1.50