Figure 4.

BP-Btz reduces bone loss and osteoblast inhibition in a mouse model of MM. Mice were described in Figure 3A. (A) Micro-CT images and data analysis of femoral bones. (B) Images of H&E-stained sections and femoral bone volume by histomorphometric analysis. (C) BM cells were cultured in the OB-inducing medium for 12 days. CFU-ALP+ colonies were identified by ALP staining. Data are means ± SD of 5–6 mice. One way ANOVA with Dunnett’s test for comparison of PBS + Veh, MM + Veh, MM + Btz, and MM + BP-Btz groups. * p < 0.05. (D) Number of paralyzed mice in each group.