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. 2018 Jul 18;6(3):42. doi: 10.3390/vaccines6030042

Figure 3.

Figure 3

In situ stimulation of MART-1 specific CD8+ T cells in melanoma sentinel lymph nodes (SLNs). Stimulation of pre-existing MART-1 specific CD8+ T cells present within metastasis negative melanoma sentinel lymph node single-cell suspensions (HLA-A2+) through ex vivo infection using either Ad5-MART-1 or Ad5/3-MART-1. (a) Combined data showing MART-126–35L Tm+ CD8+ T cells of in total 28 bulk cultures upon primary stimulation (n = 5 SLN donors) with bulk cultures from the same donor indicated with the same filling patterns. An unpaired Student t test was performed between the Ad5-MART-1 and Ad5/3-MART-1 conditions; (b) Precursor frequency of MART-126–35L Tm+ CD8+ T cells in SLN donor-5 compared to frequencies after one round of ex vivo stimulation (d10) with Ad5-MART-1 or Ad5/3-MART-1; (c) Depicted are the percentages of MART-126–35L Tm+ cells from CD8+ T cells after the primary stimulation with Ad5-MART-1 or Ad5/3-MART-1 (Day 11) and after one round of re-stimulation with irradiated MART-126–35L loaded HLA-A2+ JY cells (Day 18) (n = 2 donors 1+2, 6 bulk cultures per donor per condition). (d) Donors depicted in (c) separated for Ad5-MART-1 and Ad5/3-MART-1 stimulation showing increased or decreased frequencies of MART-1 Tm+ cells of CD8+ T cells between the primary (1st stim) and secondary (2nd stim) ex vivo stimulation. In this figure Ad5 = Ad5-MART-1, Ad5/3 = Ad5/3-MART-1.