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. 2018 Jul 10;8(3):40. doi: 10.3390/metabo8030040

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pyrimidine and the hexosamine biosynthetic pathways respond less to selenite in A549 spheroids than in their 2D cell counterparts. The same polar extracts from Figure 2 were analyzed by IC-UHRFT-MS. ¹³C fate of ¹³C₆-Glc was followed from the first turn of the Krebs cycle, PPP (pentose phosphate pathway), pyrimidine synthesis, and HBP (hexosamine biosynthetic pathway) into UTP and UDPGlcNAc (UDP-N-acetylglucosamine) along with the fractional distribution of relevant isotopologues of metabolites. ●: ¹⁴N; NCAsp: N-carbamoylaspartate; OMP: orotidine monophosphate; R5P: ribose-5-phosphate; PRPP: phosphoribosylpyrophosphate; NAcGN6P: N-acetylglucosamine-6-phosphate; NAcGN1P: N-acetylglucosamine-1-phosphate. (I) Metabolite responses in 2D cultures; (II) metabolite responses in 3D cultures. All other symbols and abbreviations are as in Figure 2.