(A) Filamentous growth of Candida albicans at 25°C. Times of incubation were as follows: YPD + serum, 9 days; Lee, 11 days; Spider, 11 days; Spider + glucose, 8 days; YPS, 3 days. (B) Filamentous growth of C. albicans at 30°C. Times of incubation were as follows: YPD + serum, 6 days; Lee, 9 days; Spider, 9 days; Spider + glucose, 5 days; YPS, 3 days. (C) Filamentous growth of C. albicans at 37°C. Times of incubation were as follows: YPD + serum, 3 days; Lee, 6 days; Spider, 6 days; Spider + glucose, 4 days; YPS, 3 days. (D) The sdh2Δ/Δ mutant cannot grow on nonfermentable carbon sources.
YPD: Contains fermentable glucose as carbon source; YPE: Contains nonfermentable ethanol as carbon source; YPG: Contains nonfermentable glycerol as carbon source.