Figure 2.

Cellulose synthase complex speed is not correlated with proximity to microtubules. (a) Particle tracking steps for CSCs in microtubule domains. Projections of 5-min time-lapse microtubule images (first panel) were used to define the microtubule domains by thresholding (green in second panel). The microtubule domain image was overlaid with CSC time-lapse images. The third panel is a single frame showing the overlay of microtubule domains (green) and YFP-CesA6 particles (white). Using spot detection plugins, CSCs were filtered from completely within the regions of interest, thus allowing the tracking of particles that remain completely within microtubule domains, as shown in the fourth panel as red. (b,c) Comparison of CSC velocity in microtubule domains versus non-microtubule domains in wild-type (b) and mor1-1 seedlings (c). Values are means ± SD. There was no significant difference in CSC velocity between microtubule and non-microtubule domains in both wild type and mor1-1. Samples were incubated at 29 °C.