The geometric mean titers (with 95% confidence interval) for previously unvaccinated subjects are compared to titers of those with records of vaccination the previous season. Results are shown for the (a) 2003 and (b) 2011 cohorts. The viruses used for testing HI titer are listed on the x axis: NC/20, A/New Caledonia/20/1999 (H1N1); MS/10, A/Moscow/10/1999 (H3N2); HK/330, B/Hong Kong/330/2001 (Victoria lineage); CA/7, A/California/07/2009 (pdmH1N1); PH/16, A/Perth/16/2009 (H3N2); and BR/60, B/Brisbane/60/2008 (Victoria lineage). The difference between the previously vaccinated group and those vaccinated in the current season was evaluated using the Mann Whitney rank test. n.s., not significant; and *, p < 0.05.