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. 2018 Sep 4;10(3):144. doi: 10.3390/pharmaceutics10030144

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of DOX and DOX-Vit D on the oxidative stress. MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D. Thereafter, total RNA was isolated using TRIzol reagent, and the mRNA levels of (A) NQO-1 and (B) HO-1 were quantified using real time-PCR and normalized to a β-actin housekeeping gene. (C) MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D then, cells were incubated with DCF-DA (10 μM) for 1 h. DCF formation was measured fluorometrically using excitation/emission wavelengths of 484/535 nm. The results are presented as the mean ± SEM (n = 6). ⁺ p < 0.05 compared to control.

Effect of DOX and DOX-Vit D on the oxidative stress. MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D. Thereafter, total RNA was isolated using TRIzol reagent, and the mRNA levels of (A) NQO-1 and (B) HO-1 were quantified using real time-PCR and normalized to a β-actin housekeeping gene. (C) MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D then, cells were incubated with DCF-DA (10 μM) for 1 h. DCF formation was measured fluorometrically using excitation/emission wavelengths of 484/535 nm. The results are presented as the mean ± SEM (n = 6). ⁺ p < 0.05 compared to control.