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. 2018 Sep 4;10(3):144. doi: 10.3390/pharmaceutics10030144

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of DOX and DOX-Vit D on MAPK signaling pathway. MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D. Thereafter, MAPKs protein phosphorylation was determined in cytoplasmic protein extracts using the PhosphoTracer (A) p38 MAPK (pT180/Y182) (B) JNK1/2/3 (pT183/Y185) (C) ERK1/2 (pT202/Y204) Elisa Kit (Abcam, Cambridge, UK). The results are presented as the mean ± SEM (n = 6). ⁺ p < 0.05 compared to control. ^* p < 0.05 compared to DOX.

Effect of DOX and DOX-Vit D on MAPK signaling pathway. MG63 cells were treated for 24 h with 10 µM DOX and DOX-Vit D. Thereafter, MAPKs protein phosphorylation was determined in cytoplasmic protein extracts using the PhosphoTracer (A) p38 MAPK (pT180/Y182) (B) JNK1/2/3 (pT183/Y185) (C) ERK1/2 (pT202/Y204) Elisa Kit (Abcam, Cambridge, UK). The results are presented as the mean ± SEM (n = 6). ⁺ p < 0.05 compared to control. ^* p < 0.05 compared to DOX.