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. 2018 Jul 2;8(3):26. doi: 10.3390/life8030026

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Oxygen protection of nitrogenase in the mutant SR652. (A) Triphenyl tetrazolium chloride (TTC) staining of WT and SR652 mutant filaments. TTC reduces to dark formazan crystals (black triangle) under micro-oxic and reducing conditions, which are necessary for the proper activity of the nitrogenase complex. Bars, 5 μm. (B) Assessment of the nitrogenase activity in the mutant SR652 and the wild type (WT) under aerobic and anoxic conditions using the acetylene reduction assay. Data are representative of two independent experiments. The histogram shows the mean values ± SD of two experimental replicates. Student’s t test P value is P < 0.02 (*).